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Omega Biotek Mag-Bind® SeqDTR

Removal of unincorporated terminators from sequencing reactions using magnetic beads

The Mag-Bind® SeqDTR is designed for efficient and reliable removal of unincorporated dye terminators from sequencing reactions. The system combines Omega Bio-tek’s proprietary chemistry with the reversible nucleic acid-binding properties of paramagnetic beads to eliminate excess nucleotides, primers, salts, and unincorporated dye terminators. This kit is designed for both manual and fully automated purification of sequencing products, and the protocol has been successfully automated on liquid handlers such as Beckman Coulter’s Biomek® FX/NX, and the Hamilton Microlab® STAR/STARlet. With the high recovery rate from the Mag-Bind® SeqDTR Kit, typically only 1/16 or 1/32 of the ABI Big Dye reagents are needed for sufficient signal strength when compared to sephadex-based clean ups.

  • No protocol change against major competitor
  • Read lengths averaging over 800 bps (Min Phred 20)
  • Manual or adjustable to automated liquid handlers
  • Significant cost savings compared to sephadex-based clean ups
  •  96- or 384-well formats
Downstream applicationSanger Sequencing, Cloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting
Elution volume40 µL or above
Starting materialABI Big Dye Chemistry Cycle Sequencing Reactions
Starting amount5 – 20 µL
DNA recoverySequencing reads start at 40 nt
Processing modeAutomated; Manual
Throughput96-384 samples per run
DNA binding technologyMagnetic beads
Binding capacityScalable
Storage2°C – 8°C

Additional information


5mLs, 50mLs, 100mLs