E.Z.N.A.® Fungal RNA Mini Kit

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Omega Biotek E.Z.N.A.® Fungal RNA Mini Kit

Isolate total RNA from fungal samples using spin columns

Modified from the E.Z.N.A.® Plant RNA Kit, the E.Z.N.A.® Fungal RNA Kit uses an improved buffer system that is more efficient for fungal samples. This kit provides convenient and rapid methods for the isolation of total RNA from a variety of fungal species. Rather, the method involves a simple and rapid precipitation step for removal of much of the polysaccharides and phenolic compounds commonly found in fungal tissues. In combination with HiBind ® RNA spin columns, this method permits purification of high-quality RNA from as much as 200 mg tissue. The system is also efficient enough to purify total RNA from as little as 10 mg of tissue or 100 cells. Purified RNA can be used for many downstream applications such as RT-PCR, Northern Blotting, Poly A +(mRNA) purification, and differential display.

Downstream ApplicationPCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification
Elution Volume50-100 μL
Starting MaterialFungal Tissue
Starting Amount<250 mg
RNA YieldUp to 100 µg, >200 nt
Processing ModeSpin column
Throughput1 – 24
RNA Binding Technology1 – 24
Binding Capacity100 µg