SKU: OME-R6954-01
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Omega Biotek E.Z.N.A.® FFPE RNA Kit

Isolates RNA from the FFPE samples using spin columns

The E.Z.N.A.® FFPE RNA Kit is specially designed for isolation of total RNA from formalin-fixed, paraffin-embedded tissue sections. This method can also be used for the preparation of total RNA from fibrous samples such as heart and muscle tissue. The sample is first processed in a special buffer by heating to deactivate RNase’s and deparaffinization. Then the lysate is treated with Proteinase K to digest proteins. The cleared lysate is then applied to a HiBind RNA column to capture RNA. After two quick wash steps, purified RNA is eluted with water. While E.Z.N.A.® FFPE RNA Kit is optimized to minimize the effect of formaldehyde modification, it is not recommended to use the RNA purified from this kit for downstream applications that require full-length RNA.

Downstream ApplicationPCR, qPCR, real-time RT-PCR, micro-array, Northern blot, poly-A purification
Elution Volume15-30 μL
Starting MaterialFFPE tissue
Starting Amount3-4 sections with a thickness of 10 μm
RNA YieldUp to 50 µg
Processing ModeManual, centrifugation
Throughput1 – 24
RNA Binding TechnologySilica Micro spin column
Binding Capacity50 µg