Description
E.Z.N.A.® FastFilter Plasmid DNA Mini Kit
The E.Z.N.A.® FastFilter Plasmid DNA Mini Kit is designed for rapid purification of high-quality plasmid DNA starting with bacterial pellet from up to 5 mL of culture following the alkaline-lysis method in just 9 minutes. The kit features an innovative and first of its kind FastFilter mini column nested within a regular HiBind® silica mini spin column to combine lysate clearance and DNA binding into one simple centrifugation step. Following wash and elute steps, purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, transformation, PCR and DNA sequencing.
Features and Benefits:
- Rapid – 9 Minute Processing time from a bacterial pellet
- Unique – Lysate Clearance and DNA Binding in 1 step
- Convenient – Lysate Clearance Column eliminates cellular debris pelleting and transfer step
- Versatile – Process up to 5 mL bacterial cultures with different plasmid types and culture medias
| FEATURES | SPECIFICATIONS |
|---|---|
| Starting Material | Bacteria harboring high-copy or low-copy plasmid in culture medium |
| Starting Amount | up to 5 mL (high-copy number plasmid) and 10 mL (low-copy number plasmid) |
| Elution Volume | 50 -100 µL |
| Processing Mode | Manual (Centrifugation or Vacuum) |
| Throughput | 1 -24 Samples |
| Nucleic Acid Binding Technology | Silica Mini Spin Column |
| Processing Time | 9 minutes |
| Downstream Application(s) | Routine screening, restriction enzyme digestion, transformation, PCR and DNA sequencing |
| Special Features | First of its kind FastFilter and HiBind® DNA mini column configuration integrating lysate clearance and DNA binding into a single step |
See more information here
