E.Z.N.A.® FastFilter Plasmid DNA Maxi Kit

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Omega Biotek E.Z.N.A.® FastFilter Plasmid DNA Maxi Kit

Rapidly isolate up to 1.2 mg plasmid DNA from 50-200 mL bacterial cultures using syringe filters for lysate clearance and maxi spin columns

The E.Z.N.A.® FastFilter Plasmid DNA Maxi Kit combines the convenience of spin columns with the speed of syringe filters to ease large-scale plasmid preparations. The system utilizes Lysate Clearance Filter Syringes instead of centrifugation to rapidly clear bacterial lysates post-alkaline lysis and follows a simple “bind-wash-elute” procedure to deliver high-quality plasmid DNA. The yields vary according to plasmid copy number, E. coli strain, and growth conditions. 50-200 mL of bacterial cultures in LB medium typically produces 0.6-1.2 mg of high copy number plasmid DNA. Up to 500 mL of culture may be processed when working with low copy number plasmid. The system uses centrifugation or vacuum technology for plasmid purification and eliminates the time-consuming gravity-flow columns that require alcohol precipitation. Purified plasmid DNA is suitable for automated fluorescent DNA sequencing (typical reads exceed 800 bp), restriction enzyme digestion, ligation, PCR, in vitro transcription, transformation, and other applications.

  • Speed – Purification of plasmid DNA < 40 minutes
  • Safety – No phenol/chloroform extractions
  • Versatile – Spin and vacuum formats available
  • High quality – DNA is suitable for a variety of downstream applications
Downstream ApplicationCloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc.
Starting material50-200 mL LB culture with OD600 between 2 and 3; or equivalent
Plasmid typeHigh-copy, low-copy, cosmid DNA
Processing modeManual (centrifugation or vacuum)
Throughput1 – 24
DNA binding technologySilica Maxi Spin Column
Lysate clearance methodFiltration using Lysate clearance syringe
Processing time<40 minutes
Yield600-1200 µg for high copy-number; 50-300 µg for low copy-number
Special noteLysate clearance syringe for rapid sample processing