Omega Biotek E.Z.N.A.® Endo-free Plasmid DNA Mini Kit I
Isolate low endotoxin (<1 EU/µg) plasmid DNA from up to 5 mL bacterial cultures using spin mini columns
Plasmid isolated with traditional purification procedures normally contains high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. Up to 5 mL of overnight cultures can be used for high copy number plasmids and 10 mL for low copy number.
The E.Z.N.A.® Endo-Free Plasmid DNA Mini Kit I integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade (<1 EU/µg) plasmid for efficient transfection. The bacterial cells are lysed using the alkaline-SDS lysis method. The cleared cell lysate is then treated with ETR reagent to efficiently remove the endotoxins. After adjusting the binding conditions, the cell lysate is applied into the HiBind® DNA column and purified DNA is eluted from the column membrane.
- Rapid – 30 minutes or less
- Safe – No phenol/chloroform extractions
- Versatile – Spin and vacuum formats available
- High-quality – Endotoxins efficiently reduced (<1 EU/µg)
|Sensitive cell line transfection; gene therapy etc.
|1-5 mL LB culture with OD600 between 2 and 3; or equivalent
|High-copy, low-copy, cosmid DNA
|1 – 24
|DNA binding technology
|Silica mini spin column
|Lysate clearing method
|15-25 µg for high copy-number; 0.1-5 µg for low copy-number
|Includes endotoxin removal step